Journal:

Article Title: Tumor necrosis factor-related apoptosis-inducing ligand induces neuronal death in a murine model of HIV central nervous system infection

doi: 10.1073/pnas.2628048100

Figure Lengend Snippet: HIV-CNS infection and apoptosis in HIV-infected hu-PBMC-NOD-SCID mice

Article Snippet: Frozen sections were incubated with Abs against human CD68 (clone PGM1), human TRAIL (clone RIK-2), IL-1β (rabbit polyclonal IgG, Endogen, Woburn, MA), human TNF-α (rabbit polyclonal IgG, Endogen), human FasL (rabbit polyclonal IgG, Santa Cruz Biotechnology), HIV-1 gag p24 (goat polyclonal IgG, ViroStat, Portland, ME), or the active form of caspase-3 (rabbit polyclonal IgG, R & D Systems).

Techniques: Infection, Virus, TUNEL Assay

Journal:

Article Title: Tumor necrosis factor-related apoptosis-inducing ligand induces neuronal death in a murine model of HIV central nervous system infection

doi: 10.1073/pnas.2628048100

Figure Lengend Snippet: Association of apoptotic cells with HIV-1-infected macrophages expressing death-inducing molecules. (a) Expression of TRAIL, TNF-α, or FasL on HIV-1-infected macrophage. hu-PBMC-NOD-SCID mice were infected with M-tropic NL-CSFV3-EGFP and LPS was given or not 7 days after infection. Three days later, brain sections were subjected to histological analysis for GFP (green), human CD68 (red), and human TRAIL, TNF-α, or FasL (blue) simultaneously. A representative cell is shown. (Scale bars, 10 μm.) (b) hu-PBMC-NOD-SCID mice were infected with JRFL, and LPS was given 7 days after infection. Three days later, brain sections were subjected to dual-color staining for human CD68, HIV gag p24, or human TRAIL (green) and TUNEL (red). (Scale bars, 100 μm.)

Article Snippet: Frozen sections were incubated with Abs against human CD68 (clone PGM1), human TRAIL (clone RIK-2), IL-1β (rabbit polyclonal IgG, Endogen, Woburn, MA), human TNF-α (rabbit polyclonal IgG, Endogen), human FasL (rabbit polyclonal IgG, Santa Cruz Biotechnology), HIV-1 gag p24 (goat polyclonal IgG, ViroStat, Portland, ME), or the active form of caspase-3 (rabbit polyclonal IgG, R & D Systems).

Techniques: Infection, Expressing, Staining, TUNEL Assay

Journal: Virology

Article Title: Epitope mapping and characterization of a novel CD4-induced human monoclonal antibody capable of neutralizing primary HIV-1 strains

doi: 10.1016/S0042-6822(03)00521-X

Figure Lengend Snippet: Fig. 1. CD4 induction of the binding of the E51 antibody to HIV-1 gp120. The YU2 HIV-1 gp120 glycoprotein was captured on an ELISA plate using the D7324 antibody, which is directed against the gp120 carboxyl-termi- nus. The binding of the 17b and E51 antibodies to the captured gp120 glycoprotein was studied in the absence (open circles) or presence (filled circles) of sCD4 (8 g/ml).

Article Snippet: The ELISA plate was coated with sheep antibody D7324 (Aalto BioReagents, Dublin, Ireland), which recognizes the gp120 carboxylterminus.

Techniques: Binding Assay, Enzyme-linked Immunosorbent Assay

Journal: International Journal of Molecular Sciences

Article Title: In Vitro Selection and Characterization of HIV-1 Variants with Increased Resistance to LP-40, Enfuvirtide-Based Lipopeptide Inhibitor

doi: 10.3390/ijms23126638

Figure Lengend Snippet: Introduction of T20- and LP40-resistant HIV-1. ( A ) The dose-escalating selections were conducted for a total of 32 passages, with T20 concentrations ranging from 500 nM to 2000 µM, and ( B ) for 32 passages, with LP40 concentrations ranging from 40 nM to 200 µM. At the indicated passage number, viral RNA was extracted from the supernatant, and gp41- and gp120-coding regions were sequenced. The mutations of gp41 are listed in the black frames. ( C ) HIV-1 NL4-3 was passaged in the presence of an increasing concentration of the inhibitors in C8166 cells.

Article Snippet: After blocking with 5% nonfat dry milk solution in Tris-buffered saline (TBS, pH 7.4) at room temperature for 1 h, the washed membrane was incubated with a rabbit anti-gp120 polyclonal antibody (SinoBiological, Beijing, China) or the human anti-gp41 monoclonal antibody 10E8 overnight at 4 °C.

Techniques: Concentration Assay

Journal: International Journal of Molecular Sciences

Article Title: In Vitro Selection and Characterization of HIV-1 Variants with Increased Resistance to LP-40, Enfuvirtide-Based Lipopeptide Inhibitor

doi: 10.3390/ijms23126638

Figure Lengend Snippet: Expression and processing profiles of HIV-1 NL4-3 Env, detected using Western blotting. The ( A ) T20- and ( B ) LP40-resistant mutant glycoproteins in the lysates of transfected cells were detected with a rabbit anti-gp120 polyclonal antibody (panel 1) and 10E8 (panel 2). The β-actin protein was detected as an internal control (panel 3). Env incorporation in ( C ) T20- and ( D ) LP40-resistant pseudoviruses was determined using immunoblotting. Concentrated virions were re-suspended in RIPA lysis buffer, then normalized to a fixed amount using P24 antigen and subjected to SDS-PAGE. A rabbit anti-gp120 polyclonal antibody (panel 4) and 10E8 (panel 5) were used to probe gp160, gp120, and gp41. As an internal control, the P24 antigen was detected with a rabbit anti-HIV P24 polyclonal antibody (panel 6). The experiments were repeated three times, and representative data are shown.

Article Snippet: After blocking with 5% nonfat dry milk solution in Tris-buffered saline (TBS, pH 7.4) at room temperature for 1 h, the washed membrane was incubated with a rabbit anti-gp120 polyclonal antibody (SinoBiological, Beijing, China) or the human anti-gp41 monoclonal antibody 10E8 overnight at 4 °C.

Techniques: Expressing, Western Blot, Mutagenesis, Transfection, Lysis, SDS Page

Journal: iScience

Article Title: Muscle glycome in idiopathic inflammatory myopathies: Impact in IL-6 production and disease prognosis

doi: 10.1016/j.isci.2023.107172

Figure Lengend Snippet:

Article Snippet: Polyclonal DC-SIGN rabbit IgG , Biorad , Cat#AHP627; RRID: AB_323683.

Techniques: Plasmid Preparation, Recombinant, Reverse Transcription, Random Hexamer, Protease Inhibitor, Staining, Isolation, Enzyme-linked Immunosorbent Assay, Software